发明名称 |
CATALYTICALLY INACTIVE PROTEINS AND METHOD FOR RECOVERY OF ENZYMES FROM PLANT-DERIVED MATERIALS |
摘要 |
An inactive xylanase molecule for the recovery of xylanase activity in plant- derived material containing active xylanase enzyme(s) and xylanase inhibitors. The inactive xylanase molecule of binds to xylanase inhibitors in the plant-derived material, thereby allowing accurate measurement of xylanase enzyme activity of the enzyme contained in the plant-derived material. The invention further includes amino acid molecules depicted by SEQ ID NOS. 4 through 112, wherein the catalytically active sites of each of the amino acids have been modified resulting in inactive xylanase molecules. A method of production of the inactive xylanase molecules includes expression of the inactive xylanase molecule in microbial or eukaryal (e.g., yeast including Pichia pastoris) host cell an expression cassette comprising a promoter operably linked to a nucleic acid molecule encoding the inactive xylanase molecule and using the expressed molecule in an assay to recover the xylanase enzyme activity in plant-derived material, for example, in plant-derived material such as animal feed. |
申请公布号 |
WO2007146944(A2) |
申请公布日期 |
2007.12.21 |
申请号 |
WO2007US71012 |
申请日期 |
2007.06.12 |
申请人 |
SYNGENTA PARTICIPATIONS AG;BAUER, MICHAEL, WILLIAM;DE FONTES, JUSTIN |
发明人 |
BAUER, MICHAEL, WILLIAM;DE FONTES, JUSTIN |
分类号 |
C12N9/24 |
主分类号 |
C12N9/24 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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