发明名称 |
EXAMINATION OF AMINO ACID SEQUENCE CONSTITUTING PEPTIDE DEPENDING ON ISOTOPIC RATIO |
摘要 |
It is an object of the present invention, when determining and identifying an amino acid sequence of a peptide using MS, to obtain additional information from the MS for evaluating validity of an amino acid sequence in a candidate list outputted from an identifying engine. The present invention provides a method of testing an amino acid sequence inferred by searching a peptide-related database based on peptide mass information and/or peptide modification information obtained through mass spectrometry on a peptide, the method comprising the steps: (1) calculating a theoretical value of an isotopic ratio for the peptide from the inferred amino acid sequence and/or the peptide modification information; (2) measuring a measured value of the isotopic ratio for the peptide from the peptide mass information; and (3) comparing the theoretical value and the measured value, and evaluating validity of the inferred amino acid sequence from differences between the theoretical value and the measured value.
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申请公布号 |
EP1775581(A1) |
申请公布日期 |
2007.04.18 |
申请号 |
EP20050768470 |
申请日期 |
2005.08.04 |
申请人 |
EISAI R&D MANAGEMENT CO., LTD. |
发明人 |
ISHIHAMA, YASUSHI;KAWAI, TAKATOSHI |
分类号 |
G01N27/62;G01N33/68 |
主分类号 |
G01N27/62 |
代理机构 |
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