MATERIALS AND METHODS FOR ASSAYING FOR GLYOXYLATE

摘要

The subject invention concerns enzyme-based methods for detecting and assaying for glyoxylate. In particular, the invention is directed to methods for assaying for glyoxylate produced by the reaction of peptidylglycine a-amidating monooxygenase (PAM). The subject invention also concerns methods for assaying for the enzyme peptidylglycine a- amidating monooxygenase. The detection of glyoxylate using the present invention is indicative of the presence of PAM. The subject invention also concerns methods for screening for peptide hormones and any N-acyl-glycine or N-aryl-glycine conjugated molecule.