| 摘要 |
<P>PROBLEM TO BE SOLVED: To provide a new DNA of xylanase, to provide the xylanase encoded with the DNA, to provide a recombinant vector in which the DNA is transduced and to provide a transformant having the recombinant vector. <P>SOLUTION: An Aureobasidium pullulans ATCC (American Type Culture Collection) 20524 strain is cultured in a liquid culture medium maintaining the pH during culture at 6 with a buffer solution containing xylan as a carbon source. Thereby, the new xylanase having 39,000 molecular weight and 8.9 isoelectric point is obtained by subjecting the resultant culture solution to various kinds of chromatographies to carry out electrophoretically simple purification. A gene encoding the enzyme is cloned by using a partial amino acid sequence obtained from the protein. An ORF (open reading frame) composed of 361 amino acid residues containing an extracellular secretory signal of 26 amino acid residues is estimated from the base sequence of the cloned DNA. It is suggested that the enzyme is the xylanase belonging to the GH family 10 by homology search of the estimated amino acid sequence. <P>COPYRIGHT: (C)2007,JPO&INPIT |
