CHIMERIC PROTEINS, CELL LINES COMPRISING SAME, AND ASSAYS FOR SCREENING PROTEINASE MODULATORS USING SAME

摘要

A chimeric protein comprising in sequence a signal peptide, a first amino acid tag, a proteinase bait, a second amino acid tag, a transmembrane domain and a cytosolic domain, wherein the cytosolic (CT) domain comprises a sequence able to recycle the protein from the cellular membrane to endosomes. A cell line expressing the chimeric protein and an assay using the cell line.