OXYGEN-RESISTANT HYDROGENASES AND METHODS FOR DESIGNING AND MAKING SAME

摘要

The invention provides oxygen-resistant iron-hydrogenases ([Fe]-hydrogenases) for use in the production of H₂. Methods used in the design and engineering of the oxygen-resistant [Fe]-hydrogenases are disclosed, as are the methods of transforming and culturing appropriate host cells with the oxygen-resistant [Fe]-hyrdogenases. Finally, the invention provides methods for utilizing the transformed, oxygen insensitive, host cells in the bulk production of H₂ in a light catalyzed reaction having water as the reactant.