METHOD FOR RETAINING PATHOGENIC TREPONEMA PALLIDUM STRAIN VIABILITY

摘要

FIELD: medicine. ^ SUBSTANCE: method involves producing pathogenic Treponema pallidum strain suspension from rabbit testis infected with Treponema pallidum. The testis is mechanically comminuted and extracted in isotonic sodium chloride solution. The suspension has native infected rabbit testis tissue extract. Then glycerol is added in the amount of 15% of total medium volume. The produced product is divided into aliquots of 0.75-1.5 ml and frozen immediately for storing it at -70 - -80°C in future after having stirred. ^ EFFECT: enhanced effectiveness in retaining infectious agent virulence.