Dna enzymes

摘要

Methods for the selection of novel signaling allosteric DNA enzymes are provided. In particular, fluorescent signaling allosteric DNA enzymes are described. The selection system is based on the cleavage of an ribonucleotide flanked by a fluorophore modified nucleotide and a quencher modified oligonucleotide. Both cis-acting and trans-acting allosteric DNA enzymes are identified, as well as aptamer/DNA enzyme conjugates.