BONE FORMATION COMPOSITION COMPOSED OF MIXTURE OF OSTEOBLAST AND BIO-MATRIX AND ITS MANUFACTURING METHOD

摘要

A bone formation composition comprising a mixture of osteoblast and bio-matrix and its manufacturing method are provided to avoid clinical rejection of its administration by administering the composition into a desired region of a subject, prevent bone formation at the undesired region induced by transferring the osteoblast into the undesired region through the blood by administering the osteoblast suspension, and reduce the time of bone formation. The method for manufacturing the bone formation composition comprises the steps of: separating osteoblasts from the tissue, culturing the osteoblasts in DMEM(Dulbecco's modified Eagle's medium) or alpha-MEM(minimum essential medium, alpha modification) to prepare the osteoblast suspension; and adding the bio-matrix into the osteoblast suspension, wherein the method further comprises a step of adding a coagulant such as thrombin and fibrinogen; and the bio-matrix is collagen or hydroxyapatite. The bone formation composition comprises the mixture of osteoblast and bio-matrix as active ingredients.