MANUFACTURED PRODUCT USING AND COLLAGEN SOLUTION MANUFACTURING METHOD AND COLLAGEN SEPARATION METHOD OF ANIMAL TISSUE

摘要

A method for separation of collagen from various animal tissues, a method for preparing a collagen solution and a product manufactured using the same are disclosed. For this purpose, the present invention provides a method for preparing a collagen solution from an animal tissue, comprising, upon final treatment of collagen: treating a collagen solution having a predetermined concentration under a neutral condition at a low temperature, followed by overnight treatment at a temperature of 30 to 35°C; concentrating collagen by centrifugation; and dissolving the thus-concentrated collagen in refrigerated weakly-acidic solvent or phosphate buffered saline (PBS), thereby preparing collagen having a concentration of 1 to 5 mg/mL. The present invention, as constituted above, enables realization of a method for efficient separation of collagen from animal bone and cartilage tissues, skin tissues and tendon/ligament tissues, a method for preparing a collagen solution from such isolated tissues, and a matrix and highly-concentrated collagen solution product using the same.