A method and apparatus of multi-dye analysis of particles using flow cytometer. The method includes dying particles to be detected using two or more dyes; urging the particles through a capillary in a non-uniform flow; exciting a first of the particles within the capillary using a multiphoton excitation laser beam causing the two or more dyes each to fluoresce thereby producing a first output signal and a second output signal respectively; and detecting the first output signal and the second output signal. A second of the particles within the capillary being excited using the multiphoton excitation laser beam causing the two or more dyes each to fluoresce thereby producing a third output signal and a forth output signal respectively. The method finally includes comparing a ratio of the first output signal and the second output signal to a ratio of the third output signal and the forth output signal to detect a desired change in the particles.
申请公布号
WO2006034046(A3)
申请公布日期
2006.09.14
申请号
WO2005US33189
申请日期
2005.09.16
申请人
THE REGENTS OF THE UNIVERSITY OF MICHIGAN;YE, JING, YONG;NORRIS, THEODORE, B.;ZHONG, CHENG, FRANK;BAKER, JAMES, R.
发明人
YE, JING, YONG;NORRIS, THEODORE, B.;ZHONG, CHENG, FRANK;BAKER, JAMES, R.