Methods for large scale production of recombinant DNA-derived tPA or K2S molecules

摘要

A method for producing recombinant DNA-derived tissue plasminogen activator (tPA) or a Kringle 2 Serine protease molecule (K2S) in prokaryotic cells, wherein the tPA or K2S is secreted extracellularly as an active and correctly folded protein comprises expressing in the cell a vector comprising the DNA coding for the tPA or K2S molecule operably linked to a DNA coding for the signal peptide OmpA. Pharmaceutical compositions comprising the tPA or K2S obtained from the method are useful in the treatment of stroke, cardiac infarction, acute myocardial infarction, pulmonary embolism, artery occlusions, deep vein thrombosis and related disease associated with unwanted blood clotting.