发明名称 |
MODIFIZIERTES E.COLI ENTEROTOXIN II SIGNALPEPTID SOWIE EIN MIKROORGANISMUS DER EIN FUSIONSPROTEIN SOWIE EIN HETEROLOGES PROTEIN EXPRIMIERT |
摘要 |
A heterologous protein is produced by: (i) culturing a microorganism transformed with an expression vector comprising a gene encoding a modified E. coli enterotoxin II signal peptide fused with the heterologous protein to produce and secrete the heterologous protein to periplasm, the modified E. coli enterotoxin II signal peptide being obtained by replacing at least one of the 2nd, 4th, 5th, 12th, 20th, and 22nd amino acids of E. coli enterotoxin II signal peptide of the following amino acid sequence (SEQ ID NO: 1) with another amino acid, with the proviso that at least one of the 2nd and 4th amino acid of the modified peptide is lysine; and (ii) recovering the heterologous protein from the periplasm. |
申请公布号 |
AT317398(T) |
申请公布日期 |
2006.02.15 |
申请号 |
AT19990944889T |
申请日期 |
1999.09.15 |
申请人 |
HANMI PHARM. CO., LTD. |
发明人 |
KWON, SE, CHANG;JUNG, SUNG, YOUB;SHIN, HOON;CHOI, JAY DO;CHOI, KI, DOO;LEE, GWAN, SUN |
分类号 |
C12N15/09;C07K14/245;C07K14/61;C07K19/00;C12N1/21;C12N15/31;C12N15/62;C12N15/70;C12P21/02;C12R1/19;(IPC1-7):C07K14/245 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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