METHOD FOR OBTAINING MESENCHYMAL STEM CELLS

摘要

The invention relates to the field of cell biology. In de­tail it relates to the obtainment of mesenchymal stem cells from human tissue. This invention might be applicable in heal­ing within the frame of the treatment of several diseases. Due to the invention it will be possible to obtain mesen­chymal stem cells from human tissue with high homogeneity of the cell suspension, since the used method for obtaining mesen­chymal stem cells from human tissue comprises the crushing and enzymatical treatment of the tissue with collagenase solution in Eagle medium in the Dulbecco modification, removal of eryth­rocytes by the aid of the lysis solution and subsequent filtra­tion of the prepared suspension; as human tissue fat tissue or decidual or amniotic placenta membrane or chorion placenta stroma is used, whereas the filtration is performed sequen­tially by the use of filters comprising a pore size of 100 pm and 10 pm. In the enzymatical treatment of the fat tissue, of the decidual or amniotic placenta membrane collagenase of the type I is used, and in the enzymatical treatment of the chorion placenta stroma collagenase of the type IV is used.