A RECOMBINANT CONSTRUCTED BY A VIRUS VECTOR AND A HUMAN TUMOR SUPPRESSOR GENE AND ITS USE

摘要

The invention relates to a recombinant virus, which is engineered to contain a human tumor suppressor gene, and its application. This recombinant constructed by DNA cloning technology is combined with virus vector and human tumor suppressor gene expression cassette, which can amplify and propagate in specific genetic-engineered cell lines, and also can express tumor suppressor protein in eukaryote cells. In this invention, the recombinant adenovirus-p53 DNA is constructed with adenoviral vector and human p53 gene by homologous recombination in prokaryotic cells (E. coli). The human p53 gene expression cassette is the specific sequence, which is composed of promoter-p53 cDNA-poly (A). The recombinant p53 adenovirus of this invention can be prepared as clinical gene therapy products, which apply in treating and preventing human malignant tumors. <IMAGE> <IMAGE>