| 摘要 |
Methods are described for attaching molecules to a solid phase, such as a magnetic bead. Reactive groups on the solid surface react with a molecule that contains a sulfhydryl group. The sulfhydryl group may, but need not be protected. The molecule is then reacted with a conjugate and ligand. The linker has a reactive group, such as a maleimido group, which reacts with sulfhydryl, and also contains a ligand, such as an antibiotic, antibody, aptamer, or other molecule of interest. The invention also involves methods for quantifying usable reactive groups on a solid phase, where the sulfhydryl group containing moiety is bound, and measured. The first step of the reaction, and preferably the second step as well, take place in non-aqueous, aprotic solvent.
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