发明名称 |
PROCESS FOR CELL CULTURING BY CONTINUOUS PERFUSION AND ALTERNATING TANGENTIAL FLOW |
摘要 |
The invention relates to a process for the culturing of cells by continuous perfusion culturing of a cell culture comprising cell culture medium and cells, wherein cell culture medium is added to the cell culture, the cell culture is circulated over a filter module comprising hollow fibers resulting in an outflow of liquid having a lower cell density than the cell culture and the flow within the filter module is an alternating tangential flow. Preferably, culture medium is added at a particular perfusion rate and/or biomass is removed form the culture at least once. The method is especially suitable for the culturing of aggregating cells. The invention also relates to such a process wherein a biological substance, preferably an antibody, is produced by the cells, which biological substance may be further purified in downstream processing. |
申请公布号 |
WO2005095578(A1) |
申请公布日期 |
2005.10.13 |
申请号 |
WO2005EP02374 |
申请日期 |
2005.03.04 |
申请人 |
DSM IP ASSETS B.V.;CROWLEY, JOHN;WUEBBEN, MAIKE;COCO MARTIN, JOSE MANUEL |
发明人 |
CROWLEY, JOHN;WUEBBEN, MAIKE;COCO MARTIN, JOSE MANUEL |
分类号 |
C12M3/06;C12N5/00 |
主分类号 |
C12M3/06 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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