| 摘要 |
PROBLEM TO BE SOLVED: To provide a quantitative method and reagent for sucrose included in a sensitive sample. SOLUTION: The quantitative method for sucrose included in a sensitive sample comprises the following steps: (a) a reaction step in whichα-D-glucose1-phosphate and fructose are generated, (b) a reaction step in whichα-D-glucose 6-phosphate is generated, (c) a reaction step in which 6-phosphogluconolacton, hydrogen ions, and thio-NADH (reduced form) or thio-NADPH (reduced form) are generated, and (d) a measurement step in which the generated thio-NADH (reduced form) or thio-NADPH (reduced form) is measured. COPYRIGHT: (C)2005,JPO&NCIPI
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