发明名称 |
СПОСОБ ВЫДЕЛЕНИЯ КОЛЛАГЕНА ИЗ МИНЕРАЛИЗОВАННОЙ СОЕДИНИТЕЛЬНОЙ ТКАНИ И КОСМЕТИЧЕСКОЕ СРЕДСТВО НА ЕГО ОСНОВЕ |
摘要 |
FIELD: chemical engineering. ^ SUBSTANCE: method involves pretreating bone tissue of stock-farm animals, comminuting it, precipitating the end product and drying it by lyophilizing. The bone tissue is degreased with alcohol-ether mixture. Demineralization 0.5 and HCl is carried out after grinding during 20 h. Non-resolvable matrix is subjected to proteolysis in HCl of pH=2.0 in pepsin presence at 37°C during 18 h. Then it is centrifuged at 40000g. The end product is precipitated from supernatant with ammonium sulfate to 25% saturation and centrifuged at 6000g. The precipitate is lyophilized against distilled water and chromatographically purified using CM-Sephadex at pH=4.8 and dried with lyophilization. ^ EFFECT: low cost collagen production; high purity cosmetic preparation production. ^ 2 dwg |
申请公布号 |
RU2003134131(A) |
申请公布日期 |
2005.05.20 |
申请号 |
RU20030134131 |
申请日期 |
2003.11.24 |
申请人 |
Государственное учреждение Российский научный центр "Восстановительна травматологи и ортопеди " им. акад. Г.А. Илизарова (RU) |
发明人 |
Лунева Светлана Николаевна (RU);Ковинька Михаил Александрович (RU);Матвеева Елена Леонидовна (RU);Талашова Ирина Александровна (RU);Накоскин Александр Николаевич (RU) |
分类号 |
(IPC1-7):A61K35/78 |
主分类号 |
(IPC1-7):A61K35/78 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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