| 摘要 |
FIELD: molecular genetics and biotechnology. ^ SUBSTANCE: invention relates to method for detection of mismatched bases (point mutations) in nucleic acids. More particularly, strategy of phage selection bearing peptide analogs of functionally active protein modules of reparation system capable to selective binding to mismatched base sites on double-stranded DNA in certain locus is disclosed. Said strategy is based on combination of phage display by using biotinylated primers to provide PCR-fragments and paramagnetic particles with streptavidine to physically separate DNA-phage complex. Selected phages are selectively (preferably) bound to mismatched base in any heteroduplex composition. Present invention provides automatic method for molecular and epidemiological investigations of various diseases. ^ EFFECT: automatic method for molecular and epidemiological investigations. ^ 4 cl, 2 dwg, 2 tbl, 4 ex |
