RECOMBINANT DNA HAVING HYDANTOINASE GENE AND CARBAMYLASE GENE AND PROCESS FOR PRODUCING AMINO ACID

摘要

The object of the present invention is to prepare a recombinant DNA efficiently co-expressing genes of enzymes such as HHase, and to produce an amino acid efficiently from hydantoin by utilizing such a recombinant DNA. The present invention provides a recombinant DNA having base sequences encoding the following gene regions (I) and (II) incorporated therein in a predetermined direction and in a predetermined order to produce an amino acid efficiently:
(I) a gene region containing a base sequence encoding a hydantoinase gene and a trp promoter sequence located upstream of the base sequence encoding the hydantoinase gene regulating expression of the hydantoinase gene, and(II) a gene region containing a base sequence encoding a carbamylase gene and a trp promoter sequence located upstream of the base sequence encoding the carbamylase gene regulating expression of the carbamylase gene.