ENHANCED EXPRESSION OF FUSION POLYPEPTIDES WITH A BIOTINYLATION TAG
摘要
<p>The invention provides the means to enhance in E.coli-based expression systems the formation of fusion polypeptides containing as an N-terminal tag a biotinylation polypeptide. By way of specifically exchanging in the nucleic acid sequence encoding the biotinylation polypeptide nucleotides at 11 discrete positions enhances the formation of the total fusion polypeptide by at least 40%.</p>
申请公布号
WO2004076670(A1)
申请公布日期
2004.09.10
申请号
WO2004EP01973
申请日期
2004.02.27
申请人
ROCHE DIAGNOSTICS GMBH;F. HOFFMANNN-LA ROCHE AG;VOGES, DIETER;WATZELE, MANFRED;WEDEKIND, FRANK