DNA VACCINE USING JAPANESE ENCEPHALITIS VIRUS AND PORCINE IMMUNOREGULATORY GENES, AND METHOD FOR USING THE SAME

摘要

PURPOSE: A DNA vaccine using Japanese encephalitis virus and porcine immunoregulatory genes, and a method for using the same are provided, which DNA vaccine selectively uses strong cellular immunity and humoral immunity by change of genetic manipulation or administration pathway, minimizes side-effects by diluents in the administration area, inhibits pathogenicity recovering shown in live vaccine, and minimizes lowering of immunity when it is used with other disease vaccines. CONSTITUTION: The DNA vaccine using Japanese encephalitis virus and porcine immunoregulatory genes comprises a recombinant plasmid pSLIA-prME(KCTC 10378BP) containing Japanese encephalitis virus gene prME and a recombinant plasmid pSLIA-NS1(KCTC 10379BP) containing porcine immunoregulatory gene NS1, and further comprises a recombinant plasmid pSLIA-PIL-2(KCTC 10380) containing porcine interleukin-2 gene PIL-2, wherein the DNA vaccine is subcutaneously administered to pigs through the pig's tail. The method for preventing the infection of Japanese encephalitis virus comprises administering the recombinant plasmids pSLIIA-prME and pSLIA-NS1 into pigs, wherein the recombinant plasmid pSLIA-PIL-2 may be further administered into pigs.