发明名称 |
DNA encoding human apoB48R: a monocyte-macrophage apolipoprotein B48 receptor gene and protein |
摘要 |
The present invention provides an isolated DNA molecule that codes for a cell-surface binding protein in human monocytes and macrophages. In addition, an amino acid sequence derived from the nucleotide sequence is provided. The newly-identified cell-surface binding protein described herein is instrumental in the apoB-mediated cellular uptake of plasma chylomicrons and remnants and hypertriglyceridemic triglyceride-rich lipoproteins in an ApoE- and lipoprotein lipase- and heparin sulfate proteoglycan-independent pathway. The new human macrophage receptor has been cloned and uniquely, binds TGRLP via apolipoprotein B48, the marker of dietary TGRLP (apoB48R). This process rapidly converts macrophages and apoB48R-transfected Chinese hamster ovary cells in vitro into lipid-filled "foam cells," hallmarks of atherosclerotic lesions. The apoB48R cDNA (3744 bp) encodes a novel protein with no known homologs. Its ~3.8 kb mRNA is expressed primarily by reticuloendothelial cells. Immunohistochemical studies indicate that foam cells of human atherosclerotic lesions express the apoB48R.
|
申请公布号 |
US6740735(B1) |
申请公布日期 |
2004.05.25 |
申请号 |
US20000583610 |
申请日期 |
2000.05.31 |
申请人 |
GIANTURCO SANDRA H.;BRADLEY WILLIAM A. |
发明人 |
GIANTURCO SANDRA H.;BRADLEY WILLIAM A. |
分类号 |
A61K38/00;C07K14/705;(IPC1-7):C07K1/00;C07H21/04;C12N1/20;C12P21/06;G01N33/506 |
主分类号 |
A61K38/00 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|