METHOD OF EXPRESSING PROTEIN HAVING UNNATURAL AMINO ACID INTEGRATED THEREINTO
摘要
<p>A method of expressing a protein having an unnatural amino acid integrated thereinto characterized by comprising expressing (A) a mutant tyrosyl tRNA synthase which is a mutant of Escherichia coli-origin tyrosyl tRNA synthase and has an elevated specificity for a unnatural tyrosine derivative compared with the specificity for tyrosine, (B) a suppressor tRNA originating in an eubacterium belonging to the genus Bacillus, Mycoplasma or Staphylococcus which is capable of binding to the above tyrosine derivative in the presence of the above mutant TyrRS, and (C) a desired protein gene having a nonsense mutation at a desired site in animal cells to thereby incorporate the above tyrosine derivative into the nonsense mutation site in the above protein.</p>
申请公布号
WO2004039989(A1)
申请公布日期
2004.05.13
申请号
WO2003JP14028
申请日期
2003.10.31
申请人
RIKEN;JAPAN SCIENCE AND TECHNOLOGY AGENCY;YOKOYAMA, SHIGEYUKI;SHIROUZU, MIKAKO;SAKAMOTO, AYAKO;SAKAMOTO, KENSAKU