METHOD OF PLASTID TRANSFORMATION IN ASTERACEAE, VECTOR FOR USE THEREIN AND PLANTS THUS OBTAINED
摘要
The present invention relates to a method for the transformation of plastid genomes of plant species, in particular Asteraceae plant species, comprising the steps of providing a transformation vector carrying a DNA sequence of interest; subjecting a plant material, which comprises plastids, to a transformation treatment in order to allow the plastids to receive the transformation vector; placing the thus treated plant material for a period of time into contact with a culture medium without selection agent; subsequently placing the plant material into contact with a culture medium comprising a selection agent; and refreshing the culture medium comprising a selection agent to allow plant material comprising plastids that have acquired the DNA of interest to grow into transformants.
申请公布号
WO2004016793(A2)
申请公布日期
2004.02.26
申请号
WO2003EP08948
申请日期
2003.08.08
申请人
RIJK ZWAAN ZAADTEELT EN ZAADHANDEL B.V.;LELIVELT, CECILIA, LUCIA, CLARA;NUGENT, JACKIE, M.;MCCABE, MATTHEW, S.;DIX, PHILIP, J.
发明人
LELIVELT, CECILIA, LUCIA, CLARA;NUGENT, JACKIE, M.;MCCABE, MATTHEW, S.;DIX, PHILIP, J.