| 摘要 |
Disclosed is a method of amplifying nucleic acids by appending a promoter sequence on an oligonucleotide and transcribing the nucleic acid. The oligonucleotide can attached to a solid phase, e. g. , a chip. In one example, nucleic acids are amplified by a method that includes: providing a first solid support having 5' attached oligonucleotide; annealing a complex sample that comprises sample nucleic acids to the solid support; and producing template nucleic acids immobilized on the solid support that each include at least a segment of the sample nucleic acids, such that the immobilized templates represent the composition of the sample nucleic acids. |
