发明名称 |
MULTI-WELL MICROFILTRATION APPARATUS |
摘要 |
<p>The present invention provides multi-well plates and column arrays in which samples (e.g., cell lysates containing nucleic acids of interest, such as RNA) can be analyzed and/or processed. In one embodiment, the microfiltration arrangement is a multilayer structure, including (i) a column plate having an array of minicolumns into which samples can be placed, (ii) a discrete filter element disposed in each minicolumn, (iii) a drip-director plate having a corresponding array of drip directors through which filtrate may egress, and (iv) a receiving-well plate having a corresponding array of receiving wells into which filtrate can flow. The invention provides multi-well microfiltration arrangements that are relatively simple to manufacture and that overcome many of the problems associated with the prior arrangements relating to (i) cross-contamination due to wicking across a common filter sheet or (ii) individual filter elements entrapping sample constituents within substantial dead volumes. Further, the invention provides multi-well microfiltration arrangements that adequately support discrete filter elements disposed in the wells without creating substantial preferential flow. Additionally, the invention provides multi-well microfiltration arrangements that avoid cross-contamination due to aerosol formation, pendent drops and/or splattering. Other disclosed features of the invention provide for the automated covering or heat-sealing of filtrate samples separately collected in an array of wells. <IMAGE></p> |
申请公布号 |
EP1124637(B1) |
申请公布日期 |
2004.01.02 |
申请号 |
EP19990957491 |
申请日期 |
1999.10.28 |
申请人 |
APPLERA CORPORATION |
发明人 |
MORING, STEPHEN, E.;BODNER, KEVIN, S.;HALSEY, JASON, H.;HOSHIZAKI, JON;JACKSON, JOSEPH;MADDEN, ALFRED, P.;OLDHAM, MARK, F.;REED, MARK, T. |
分类号 |
G01N1/00;B01D29/00;B01D35/02;B01D53/22;B01D61/18;B01L3/00;C12N15/09;G01N35/00;G01N35/02;G01N35/10;(IPC1-7):B01L3/00;B65B7/28;B65D1/34 |
主分类号 |
G01N1/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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