| 摘要 |
FIELD: molecular biology, biochemistry. SUBSTANCE: invention relates to method for evaluation of damage of DNA. Represented method involves preparing control double- stranded DNA-matrix with first and second complementary chains being each chain has chain-specific primer site and primer sites are divided by at least 500 base pairs. Linkage of first and second complementary chain with primers. Realization of quantitative amplification using rTth-polymerase and Tricine-buffer. Repeating above indicated stages in testing double-stranded DNA-matrix subjected for effects by conditions that damage DNA. Evaluation of DNA damage by assay of reduction of amplification degree for testing DNA-matrix with respect to control DNA-matrix. Method allows carrying out analysis of DNA of length above 5 000 base pairs and to carry out control of genetic damages occurring as result of self-inducing effect of gene toxins. EFFECT: improved evaluation method. 6 cl, 4 tbl, 28 dwg, 11 ex
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