发明名称 Methods for producing high yields of zygotic-like cotyledonary pine embryos utilizing media that include a disaccharide and glucose.
摘要 FIELD: agriculture. ^ SUBSTANCE: embryogenetic pine tissue is cultivated in or on the medium containing maltose and glucose, for cultivation of pine cotyledonous blastemals. Both maltose and glucose are available in the medium at concentration less than 3%. The maltose concentration in medium is 2.5 times higher than concentration of glucose. In one of the versions of this method the pine tissue is first kept in the medium for support of animated existence, and the mentioned concentrations of maltose and glucose are used further for cultivation in the medium for development from embryogenetic tissue of cotyledonous blastemals of a pine. ^ EFFECT: productivity increase of cotyledonous blastemals of a pine. ^ 26 cl, 2 dwg, 2 tbl, 2 ex
申请公布号 ZA200302462(B) 申请公布日期 2003.10.08
申请号 ZA20030002462 申请日期 2003.03.28
申请人 WEYERHAEUSER COMPANY 发明人 PRAMOD K. GUPTA;BONNIE LARSON;DIANE HOLMSTROM
分类号 A01G;A01H;C12N 主分类号 A01G
代理机构 代理人
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