AVIDIN DIMERS EFFECTIVE IN INCREASING THE CONCENTRATION OF RADIOACTIVE BIOTIN IN PRETARGETED RADIOIMMUNOTHERAPY

摘要

Dimers of avidin and streptavidins (diavidins) are described wherein the linker is suberate, which in turn, is bound to different functional groups ( - NH2 o-COOH) of avidin. As compared to avidin, the diavidins have shown the ability to increase the amount of labelled biotin on the target, when used i n an in vitropretargeting test using supported human tenascin, the biotinylate d anti-tenascin monoclonal antibody (Mab-B), avidin/diavidin, and biotin-3H. T he use of such diavidins is also described in cancer diagnosis and anticancer therapy based on the three-step pretargeted radioimmunotherapy procedure.