发明名称 |
Polycistronic expression of antibodies |
摘要 |
Described herein is a novel expression system for producing multiple gene products of interest from a single polycistronic construct. In particular, the expression system contains a polycistronic vector capable of expressing functional antibodies in eukaryotic host cells, which vector contains at least the following elements operably linked in the 5' to 3' orientation: a promoter operable in a eukaryotic cell; a DNA sequence encoding at least the variable region of an antibody light chain; an internal ribosome entry site (IRES); and at least one DNA sequence encoding an antibody heavy chain. Also disclosed are mammalian cells containing the polycistronic expression vector, and a method of producing functional antibodies in mammalian cells transfected with the polycistronic expression vector. |
申请公布号 |
US2003157641(A1) |
申请公布日期 |
2003.08.21 |
申请号 |
US20020295823 |
申请日期 |
2002.11.18 |
申请人 |
IDEC PHARMACEUTICALS CORPORATION |
发明人 |
REFF MITCHELL;BARNETT RICHARD |
分类号 |
A61K47/48;A61K51/10;C07K16/00;C07K16/28;C07K16/30;C12N15/85;C12P21/02;(IPC1-7):C12P21/02;C12N1/18;C12N15/86;C12N5/06;C12N15/74 |
主分类号 |
A61K47/48 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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