发明名称 |
Method for easy cloning and selection of chimeric DNA molecules |
摘要 |
The invention provides a method that allows the construction of a chimeric and/or modified and/or reconstructed DNA molecule from two DNA fragments in a defined order and orientation, and to clone the molecule one step in a suitable vector using site specific recombination. No initial step of classical cloning via restriction enzymes is needed, in contrast to the classical recombination systems. This method allows the reliability of the recombination method for cloning with the flexibility of PCR to introduce modifications in the insert sequence. Moreover, this method allows the construction of chimerical DNA molecules associating two different elements, such as promoter-gene association or fusion proteins.
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申请公布号 |
US2003143618(A1) |
申请公布日期 |
2003.07.31 |
申请号 |
US20030349782 |
申请日期 |
2003.01.23 |
申请人 |
HATZFIELD YVES;FRANKARD VALERIE MARIE-NOELLE;DROUAL ANNE-MARIE |
发明人 |
HATZFIELD YVES;FRANKARD VALERIE MARIE-NOELLE;DROUAL ANNE-MARIE |
分类号 |
C12N9/02;C12N15/66;(IPC1-7):C12Q1/68;C12P19/34 |
主分类号 |
C12N9/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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