Method of detecting the release of substances from cell organelles by means of flow cytometry

摘要

The invention is directed to a method of detecting the release of substances from cell organelles by means of flow cytometry, comprising the steps of: fixing cells to be studied; permeabilizing the outer cell membrane; staining the cells with a fluorochrome compound which binds specifically to at least one substance to be studied; measuring the individual cells by means of flow cytometry while stimulating the fluorochrome compound; and determining the fluorescent strength of the cells.