发明名称 |
Cell-free replication system and uses thereof |
摘要 |
The invention relates to improvements in cell-free systems for initiating DNA replication under cell cycle control. The system comprises a synchronous population of G1 nuclei which have been released from G0; and S phase cytosol. In a preferred aspect, a polypeptide is supplied to the system, wherein the polypeptide is Cdc6 and/or at least one MCM protein. The system is suitable for DNA replication assays, for example to test substances which modulate DNA replication.
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申请公布号 |
US6521405(B1) |
申请公布日期 |
2003.02.18 |
申请号 |
US20000486610 |
申请日期 |
2000.03.09 |
申请人 |
CANCER RESEARCH CAMPAIGN TECHNOLOGY LIMITED |
发明人 |
LASKEY RONALD;WILLIAMS GARETH HAYDN;KRUDE TORSTEN;STOEBER KAL;MILLS ANTHONY DAVID |
分类号 |
C07K14/47;C12N9/12;C12P19/34;(IPC1-7):C12Q1/68;C12N1/38;C12N5/00 |
主分类号 |
C07K14/47 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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