发明名称 |
Enzyme/tag binding and detection system |
摘要 |
A hybrid polypeptide composed of an identification peptide and a desired functional protein are produced by recombinant DNA techniques. A DNA expression vector is constructed that includes segments of DNA coding for the identification peptide and the desired functional protein. The identification peptide consists of a specific sequence of amino acids that has the desired affinity for a non-antibody capture protein. This peptide maybe linked to either the amino or carboxyl terminal of the functional protein. The identification peptide may or may not contain a sequence of amino acids that can be fragmented by sequence specific proteases or chemical agents to yield the native protein. The hybrid polypeptide produced by either cell or cell-free based expression systems is now suitable for further processing. This fusion protein can be purified by affinity chromatographic techniques using an immobilized non-antibody capture protein that has the desired affinity for the identification peptide. Alternately the capture protein may contain a label that will allow for the tracking of the functional protein in the system being studied.
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申请公布号 |
US2003032146(A1) |
申请公布日期 |
2003.02.13 |
申请号 |
US20010901996 |
申请日期 |
2001.07.10 |
申请人 |
DWULET FRANCIS EDWARD;BALGOBIN NEIL GWYN;MCCARTHY ROBERT CRANE |
发明人 |
DWULET FRANCIS EDWARD;BALGOBIN NEIL GWYN;MCCARTHY ROBERT CRANE |
分类号 |
C12N15/09;C07K1/36;C07K14/81;C12P21/02;C12P21/04;C12P21/06;(IPC1-7):C12P21/04;C07K1/22 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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