APPARATUS FOR ELECTROPHORESIS SEPARATION ON MICROCHANNELS AND FOR LASER-INDUCED FLUORESCENCE DETECTION

摘要

The invention concerns an integrated apparatus (1) comprising a miniaturised flattened support (2) having a planar surface (3) whereon are formed wells (23, 32, 34) and a microchannel (20), means for projecting (12) exciting light locally on an excitation zone of the microchannel along a direction forming an angle ( alpha ) greater than 60 DEG with a longitudinal direction of the microchannel, optical collector means (7) coupled with one free end of the microchannel, optical measurement means (8) and processing means (9), the microchannel having a portion forming a reservoir with internal cross-section flaring from its free end up to said excitation zone, said portion being extended by a conical, ellipsoidal or paraboloidal internal wall, and emerging onto a second microchannel portion with reduced internal cross-section, the latter communication with said wells.