| 摘要 |
PROBLEM TO BE SOLVED: To provide a model nonhuman animal capable of developing the disease caused by the damage of an α1 B Ca<2+> channel function, useful for the elucidation of various kinds of pain-transferring mechanisms, and for a method for preventing and treating diseases such as neurodegenerative disorder, endogenous pain, chronic pain, autonomic nervous system disease and temporary emotional disorder, and further to provide a method or the like for screening a prophylactic and therapeutic agent of the disease by using the model nonhuman animal. SOLUTION: This model nonhuman animal developing the disease caused by the damage of the α1 B Ca<2+> channel function is produced by substituting the whole or a part of a gene fragment of a potential-dependent Ca<2+> channel α1 B gene obtained from a mouse gene library with a marker gene to construct a targeting vector, making the target vector adenose, transducing the resultant target vector to an embryo stem cell, microinjecting the target embryo stem cell lacking the potential-dependent Ca<2+> channel α1 B gene function to the blastocyst of a mouse, and returning the resultant blastocyst to an adoptive parent. |
