发明名称 |
METHODS FOR PURIFYING NUCLEIC ACIDS |
摘要 |
Methods are provided for producing highly purified compositions of nucleic acids by using a highly streamlined and readily automated process. The methods use static mixers for lysing cells and precipitating debris, followed by centrifugation and ion exchange chromatography. The process may include a purification step using tangential flow ultrafiltration. A scaleable process for producing pharmaceutical grade plasmid DNA, useful for gene therapy, is provided.
|
申请公布号 |
US2002198372(A1) |
申请公布日期 |
2002.12.26 |
申请号 |
US19980121798 |
申请日期 |
1998.07.23 |
申请人 |
BRIDENBAUGH ROBERT;DANG WARREN;BUSSEY LEE B. |
发明人 |
BRIDENBAUGH ROBERT;DANG WARREN;BUSSEY LEE B. |
分类号 |
C12N15/00;A61K31/70;A61K31/7088;A61K31/7105;A61K31/711;A61K38/00;B01D15/08;B01D61/14;B01F3/08;B01F5/00;B03B5/28;C07H1/06;C07H21/00;C07H21/04;C12N15/09;C12N15/10;C12P19/34;(IPC1-7):C07H21/04 |
主分类号 |
C12N15/00 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|