A METHOD OF DETECTING BIOLOGICALLY ACTIVE SUBSTANCES

摘要

The present invention relates to a method of detecting a biologically active substance affecting intracellular processes, the method comprising: (a) culturing a cell containing a DNA sequence coding for (i) a green fluorescen t protein (GFP) having a protein kinase recognition site, or (ii) a green fluorescent protein wherein one or more amino acids have been substituted, inserted or deleted to provide a binding domain of a second messenger or an enzyme recognition site, or (iii) a hybrid polypeptide of green fluorescent protein (GFP) or a modified GFP and an enzyme recognition site or a binding domain for a second messenger under conditions permitting expression of the DNA sequence; (b) measuring the fluorescence of the cell; (c) incubating the cell with a sample suspected of containing a biologically active substance affecting intracellular processes; and (d) measuring the fluorescence produc ed by the incubated cell and determining any change in the fluorescence compare d to the fluorescence measured in step (b), such change being indicative of th e presence of a biologically active substance in said sample.