METHOD FOR DETERMINING THE PRESENCE OF EXTENSION PRODUCTS

摘要

The present invention relates to a method and reagents for determining extension products in reactions involving nucleic acid chain extension, e.g., in cyclic minisequencing, and to the use of this method when identifying specific point mutations and genetic variations. Three standard unmodified primers are needed and a non-specific fluorescent reagent (e.g. a ssDNA-binding dye) can be used as one of the fluorophores in the FRET assay used. The invention is also related to vertical light beam fluorometry.