PURPOSE: Provided is a method for checking if DNA is amplified after PCR(polymerase chain reaction) by observing the change of color by adding ferrous 1,10-phenanthroline to a PCR solution and for ascertaining the concentration of the amplified DNA. CONSTITUTION: The method comprises the steps of: performing the PCR; adding 1 microliter of the ferrous 1,10-phenanthroline to the PCR solution; ascertaining the change of color of the PCR solution by leaving the PCR solution alone at a temperature of 10-90deg.C for 30 seconds to 10 minutes and observing, wherein the change of color is ascertained by the naked eye or using absorptivity.
申请公布号
KR20020076381(A)
申请公布日期
2002.10.11
申请号
KR20010016171
申请日期
2001.03.28
申请人
LEE, DONG HUN;LEE, KI SEI
发明人
CHO, JANG CHEON;JANG, DEOK JIN;KIM, GYEONG JU;LEE, DONG HUN;LEE, GYU HO;LEE, KI SEI