IDENTIFICATION OF NUCLEOTIDE SEQUENCE SPECIFIC TO MYCOBACTERIUM AND DEVELOPMENT OF DIFFERENTIAL DIAGNOSIS STRATEGY FOR MYCOBACTERIAL SPECIES

摘要

PROBLEM TO BE SOLVED: To provide a method for detecting non-tuberculous Mycobacterium strains(NTM) in a sample. SOLUTION: This method comprises the steps of (i) providing a non- tuberculous Mycobacterium species-specific upstream p34 gene region (us-p34) nucleotide probe, (ii) reacting the us-p34 nucleotide probe with the above sample under conditions that allow for the selective formation of nucleotide duplexes between the us-p34 nucleotide probe and the corresponding no-tuberculous Mycobacterium nucleic acid target present in the sample, and (iii) detecting any nucleotide duplexes containing the p34 nucleotide probe.