IMMUNO-PCR KIT FOR SEROLOGICAL DIAGNOSIS OF GASTRIC CARCINOMA

摘要

PURPOSE: An immuno-PCR kit for serological diagnosis of gastric carcinoma is provided, thereby the gastric carcinoma can be simply and early diagnosed by detecting carcinoma-associated antigens. CONSTITUTION: The single determinant immuno-PCR kit for serological diagnosis of gastric carcinoma is capable of detecting carcinoma-associated antigens being in the human serum. Wherein Avidin is used as a binding protein which binds a monoclonal antibody with DNA; the monoclonal antibody and DNA are biotinylated; pXJ 19 is used as a marker DNA; primers used for PCR have the nucleotide sequence of SEQ ID NOs: 1 and 2; the monoclonal antibody is MG7-Ag; and the carcinoma may further contains esophagus cancer, colon cancer, liver cancer, ovarian cancer, uterine cancer and lung cancer.