PROCESS FOR PREPARING A HUMAN PAPILLOMAVIRUS VACCINE FOR THE ADMINISTRATION TO HUMANS

摘要

The invention relates to a process for preparing human Papillomavirus vaccine for the administration to humans: (a) transforming the yeast with a DNA molecule, said DNA molecule encoding the human Papillomavirus proteins L1 or the human Papillomavirus L1 +L2 proteins with the production of a transformed yeast cell (b) cultivating the transformed cell under conditions that permit the production of recombinant proteins and the spontaneous assembling thereof into virus-like particles, (c) harvesting the virus-like particle from the transformed cell (d) purifying the virus-like particles by at least one chromatographic stage and (e) conditioning the active principle which can be comprise a single type of virus-like particles or a mixture of virus-like particles, namely virus-like particles from human Papillomavirus 11, human Papillomavirus 16 and human Papillomavirus 18, so that the vaccine be subjected to oral, subcutaneous, on the mucous membrane, topical or intramuscular administration, the active principle being optionally adsorbed by Al(OH)3 at a concentration of up to 100 micro g/ ml.