| 摘要 |
This invention pertains to solid-phase oligosynthesis, and more particularly to universal solid supports for solid-phase oligonucleotide synthesis, methods for their preparation, and methods for their use. One aspect of the invention pertains to a method for the preparation of a universal solid support suitable for use in solid-phase oligosynthesis, which method comprises the steps of: (a) reacting a pendant functional group (e.g., -NH2) of a solid support (e.g., CPG) with a linker reagent (e.g., oxalyl chloride), thereby forming a pendant linker group (e.g., -C(=O)Cl); (b) removing at least a portion of excess unreacted linker reagent by evaporation (e.g., using a rotavapor apparatus); and, (c) reacting said pendant linker group with a cyclic reagent (e.g., protected inosine), thereby forming a pendant cyclic group. |
