发明名称 |
ENZYME-LINKED IMMUNO-MAGNETIC ELECTROCHEMICAL BIOSENSOR |
摘要 |
A electrochemical biosensor system based on enzyme-linked immuno-magnetic sandwich assay wherein an interdigitated array of electrodes is equipped with a magnet to attract magnetic beads. Magnetic particles bear a first recognition molecule capable of binding to the analyte. Enzymes are chemically modified to complex with the analyte. When the sandwich assay is performed, a substrate is added. The substrate is chosen such that it is cleavable by the enzyme in a reporting molecule capable of redox recycling. A substrate when cleaved preferably leads to a p-aminophenol.
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申请公布号 |
WO0047983(A9) |
申请公布日期 |
2001.08.30 |
申请号 |
WO2000US03485 |
申请日期 |
2000.02.10 |
申请人 |
UNIVERSITY OF SOUTHERN CALIFORNIA;MACPHEE, ROBERT, D. |
发明人 |
MACPHEE, ROBERT, D. |
分类号 |
G01N33/53;C12M1/34;C12Q1/34;C12Q1/42;C12Q1/44;G01N27/327;G01N33/543;(IPC1-7):G01N27/327;C12Q1/00 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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