发明名称 |
Recombinant hepatitis C virus RNA replicase |
摘要 |
A recombinant RNA-dependent RNA polymerase of hepatitis C virus (r-HCV-RDRP) coding DNA was cloned and expressed yielding active enzyme in vitro. The r-HCV-RDRP can include up to 20 added amino acids and up to nine deleted or substituted amino acids at the NH2-terminus of the encoded amino acid sequence. The invention provides method to solubilize r-HCV-RDRP from a host cell lysate and purified r-HCV-RDRP. Methods for screening for inhibitors of r-HCV-RDRP in vitro, for making stably transfected mammalian cells expressing r-HCV-RDRP and for in vivo testing of r-HCV-RDRP inhibitors in vivo are disclosed. The invention provides antibodies to r-HCV-RDRP and methods for detecting antibodies to HCV-RDRP in serum of human patients.
|
申请公布号 |
US6248589(B1) |
申请公布日期 |
2001.06.19 |
申请号 |
US19990337028 |
申请日期 |
1999.06.25 |
申请人 |
EMORY UNIVERSITY |
发明人 |
HAGEDORN CURT H.;AL REINOLDUS H. |
分类号 |
G01N33/576;C07K16/40;C12N1/21;C12N5/10;C12N9/12;C12N15/09;C12N15/54;C12Q1/48;C12R1/19;C12R1/91;(IPC1-7):C12N15/10 |
主分类号 |
G01N33/576 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|