发明名称 |
IMMUNOLOGICAL DETECTION OF RNA:DNA HYBRIDS ON MICROARRAYS |
摘要 |
The present invention provides a hybridization method and kit for the detection and measurement of biological molecules. A test sample containing the biological molecules of interest is hybridized with an unlabeled or optionally a detectably labeled complementary biomolecule to form a double-stranded hybrid immobilized to a solid phase. The immobilized hybrid may be detected with an entity which specifically recognizes an RNA:DNA hybrid, followed by analyses and quantification. Therefore, the present invention provides a method and kit to detect and measure biological molecules that is simple to use, highly specific, sensitive, and accurate for screening a plurality of biological molecules.
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申请公布号 |
WO0136681(A2) |
申请公布日期 |
2001.05.25 |
申请号 |
WO2000US31277 |
申请日期 |
2000.11.14 |
申请人 |
DIGENE CORPORATION |
发明人 |
LAZAR, JAMES, G.;ZAKEL, JOAN, M.;STRANGE, CHRISTINA, M.;WILLIAMS, INNA, R.;LORINCZ, ATTILA, T. |
分类号 |
G01N31/22;C07K16/44;C12N15/09;C12N15/11;C12Q1/68;G01N33/53;G01N33/543;G01N33/577;G01N37/00;(IPC1-7):C12Q1/68 |
主分类号 |
G01N31/22 |
代理机构 |
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地址 |
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