发明名称 |
METHODS FOR IMPLANTING CELLS |
摘要 |
A method for implanting cells onto a prosthesis includes the steps of: (a) providing a prosthesis including a porous tube, where at least 25% of the pores on the inner surface of the tube have diameters of more than about 40 mum, at least 25% of the pores on the outer surface of the tube have diameters of less than about 30 mum, and the tube includes a substantially continuous layer of a biocompatible material; (b) contacting the prosthesis with a suspension of cells; and (c) providing a pressure differential between the inner surface and the outer surface, whereby the cells are retained in the pores of the inner surface. A method for obtaining an endothelial cell culture from a blood sample involved:(a) obtaining a sample for mononuclear cells from a blood sample; and (b) culturing the sample of mononuclear cells, without further cell separation, on a cell adhesive polymer-coated solid support in the presence of endothelial growth factors. |
申请公布号 |
EP1095132(A1) |
申请公布日期 |
2001.05.02 |
申请号 |
EP19990938724 |
申请日期 |
1999.07.08 |
申请人 |
BRIGHAM & WOMEN'S HOSPITAL, INC. |
发明人 |
DZAU, VICTOR, J.;PRATT, RICHARD;MANN, MICHAEL, J.;EHSAN, AFSHIN;GRIESE, DANIEL P. |
分类号 |
C12N11/04;A61F2/00;A61F2/06;A61K35/12;A61L27/38;A61L27/50;A61L27/56;A61P9/00;C12N5/00;C12N5/071;(IPC1-7):C12N5/00;C12N5/08 |
主分类号 |
C12N11/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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